Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human IGF-1R around the phosphorylation site of tyrosine 1280 (S-F-Y(p)-Y-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08069
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
IGF-1R(phospho-Tyr1280) antibody detects endogenous levels of IGF-1R only when phosphorylated at tyrosine 1280.
References:
Morrione A, et al. (2000) Cancer Res. 60(8):2263-72.
O'Connor R, et al. (1997)Mol Cell Biol. 1997 Jan;17(1):427-35.
Western blot analysis of extract from 293 cells untreated or treated with insulin using IGF-1R (phospho-Tyr1280) antibody (E011302).