• Catalog Number:

  E011301-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GSK3β around the phosphorylation site of tyrosine 216 (V-S-YP-I-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49841

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  GSK3β (Phospho-Tyr216) antibody detects endogenous levels of GSK3β only when phosphorylated at tyrosine 216.

• References:

  Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137(1-2): 193-201 Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316(2): 411-415 Fan G, et al. J Biol Chem 2003 Dec 26; 278(52): 52432-52436 Liao X, et al. Mol Cancer Ther 2003 Nov; 2(11): 1215-1222

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK3β (Phospho-Tyr216) antibody (E011301).

Western blot analysis of extract from 293 cells, untreated or treated with insulin (40µM, 30min), using GSK3β (Ab-216) Antibody (E021301, Lane 1 and 2) and GSK3β (Phospho-Tyr216) Antibody (E011301, Lane 3 and 4).