• Catalog Number:

  E1A8046A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Merlin around the phosphorylation site of serine 10 (R-M-SP-F-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P35240

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  MERL; moesin-ezrin-radizin-like protein; neurofibromatosis type 2 gene; neurofibromatosis-2; Neurofibromin 2; NF-2; NF2; SCH; Schwannomerlin; schwannomerlin; Schwannomin

• Specificity Sensitivity:

  Merlin (Phospho-Ser10) antibody detects endogenous levels of Merlin only when phosphorylated at serine 10.

• References:

  Trofatter J.A., Cell 72:791-800(1993). Rouleau G.A., Nature 363:515-521(1993). Zucman-Rossi J., Hum. Mol. Genet. 7:2095-2101(1998).

• All Sites:

  Human: Ser10; Mouse: Ser10; Rat: Ser6

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Merlin (Phospho-Ser10) antibody. Western blot analysis of extracts from 3T3 cells, treated with IFN (2500U/ml, 30mins), using Merlin (Phospho-Ser10) antibody.