• Catalog Number:

  E011287-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CaMKII around the phosphorylation site of threonine 286 (Q-E-TP-V-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UQM7

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  CaMKII (phospho-Thr286) antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 286.

• References:

  Pak JH, et al. Proc Natl Acad Sci U S A. 2000 Oct 10; 97(21): 11232-11237 Hudmon A, et al. J Cell Biol. Author manuscript; available in PMC 2006 May 7 Miller P, et al. PLoS Biol. 2005 Apr; 3(4): e107 Runyan JD, et al. Learn Mem. 2005 Mar; 12(2): 103-110.

Western blot analysis of extract from Mouse brain tissue, using CaMKII (Ab-286) antibody (E021279, Lane 1 and 2) and CaMKII (phospho-Thr286) antibody (E011287, Lane 3 and 4).