• Catalog Number:

  E011278-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  O60496

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  p56Dok-2 (phospho-Tyr299) antibody detects endogenous levels of p56Dok-2 only when phosphorylated at tyrosine 299.

• References:

  Feng Cong, et,al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325. Serge Lemay, et,al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754. Ute Schaeper, et,al.(2000) J. Cell Biol ; 149: 1419. Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using p56Dok-2 (phospho-Tyr299) antibody (E011278).

Starvation - - - + Peptide - + - - Western blot analysis of extracts from K562 cells, using p56Dok-2 (Ab-299) antibody (E021270, Line 1 and 2) and p56Dok-2 (phospho- Tyr299) antibody (E011278, Line 3 and 4).