Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human EPS15 around the phosphorylation site of tyrosine 849.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human; Mouse; Rat
SwissProtNo:
P42566
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Other Names:
AF-1P; AF1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS15; MLLT5; Protein AF-1p; Protein Eps15
Specificity Sensitivity:
EPS15 (Phospho-Tyr849) antibody detects endogenous levels of EPS15 only when phosphorylated at tyrosine 849.
References:
Wong W.T., Oncogene 9:1591-1597(1994). Bernard O.A., Oncogene 9:1039-1045(1994).
Bernard O.A., Oncogene 9:1039-1045(1994).
All Sites:
Human: Tyr849; Mouse: Tyr849; Rat: Tyr850
Western blot analysis of extracts from HepG2 cells, using EPS15 (Phospho-Tyr849) antibody.