• Catalog Number:

  E011273-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 129 (I-L-SP-R-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16220

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  CREB (phospho-Ser129) antibody detects endogenous levels of CREB only when phosphorylated at serine 129.

• References:

  Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948. Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543. Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674. Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (phospho-Ser129) antibody (E011273).

Western blot analysis of extracts from 293 cells untreated or treated with UV, using CREB (Ab-129) antibody (E021265, Lane 1 and 2) and CREB (phospho-Ser129) antibody (E011273, Lane 1 and 2).