• Catalog Number:

  E1A1161A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of serine 328 (R-N-SP-V-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P14598

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, P47 phox, p47-phox

• Specificity Sensitivity:

  Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 328.

• References:

  Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989). Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989). Lomax K.J., Science 245:409-412(1989).

• All Sites:

  Human: Ser328; Mouse: Ser329; Rat: Ser329

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody. Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.