Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1110 (P-V-YP-H-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P00533
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
EGFR (phospho-Tyr1110) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 1110.
References:
Kaisa Erjala, et,al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111.
Judit Anido, et,al. (2003) Clin. Cancer Res ; 9: 1274.
Julian Andreev, et,al. (2001) J. Biol. Chem ; 276: 20130 - 20135.
Suil Kim, et,al. (2002) Am J Physiol Lung Cell Mol Physiol, ; 283: 67.