• Catalog Number:

  E1A1141A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDK5 around the phosphorylation site of tyrosine 15 (G-T-YP-G-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q00535

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  CDK5, CRK6, Cell division protein kinase 5, EC 2.7.11.22, PSSALRE, TAU protein kinase II catalytic subunit, TPKII catalytic subunit, Tau protein kinase II catalytic subunit, kinase Cdk5

• Specificity Sensitivity:

  CDK5 (Phospho-Tyr15) antibody detects endogenous levels of CDK5 only when phosphorylated at tyrosine 15.

• References:

  Meyerson M., EMBO J. 11:2909-2917(1992). The MGC Project Team; Genome Res. 14:2121-2127(2004). Honma N., Biochem. Biophys. Res. Commun. 310:398-404(2003).

• All Sites:

  Human: Tyr15; Mouse: Tyr15; Rat: Tyr15

Western blot analysis of extracts from COS7 cells (line 1) treated with EGF (200ng/ml, 30mins) and 293 cells (line 2) treated with H2O2 (100μM, 15mins), using CDK5 (Phospho-Tyr15) antibody.