Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human GluR1 around the phosphorylation site of serine 849 (Q-Q-SP-I-N ).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P42261
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
GluR1(phospho-Ser849) Antibody detects endogenous levels of GluR1 only when phosphorylated at serine 849.
References:
Emamian ES, et al. (2004) J Neurosci. 24(7): 1561-4
Palmer, C.L. et al. (2005) Pharmacol. Rev. 57, 253-277.
Western blot analysis of extract from mouse brain tissue, using GluR1 (Ab-849) antibody (E021253, Lane 1 and 2) and GluR1 (phospho-Ser849) antibody (E011261, Lane 3 and 4).