• Catalog Number:

  E012002-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Abl1 around the phosphorylation site of tyrosine 412 (D-T-YP-T-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00519

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  Abl1 (phospho-Tyr412) antibody detects endogenous levels of Abl1 only when phosphorylated at tyrosine 412.

• References:

  Wang J.Y. et al. (2000) Oncogene 19, 5643-5650. Danial N.N. et al. (2000) Oncogene 19, 2523-2531. Pluk H. et al. (2002) Cell 108, 247-259. Rikova K, et al. (2007) Cell 131, 1190-203. Goss VL, et al. (2006) Blood 107, 4888-97.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Abl1 (phospho- Tyr412) antibody (E012002).

Western blot analysis of extracts from COS7 cells treated with Adriamycin. Left: Using Abl1 (phospho- Tyr412) antibody (E012002). Right: Using the same antibody preincubated with synthesized peptide.