• Catalog Number:

  E011258-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Connexin43 around the phosphorylation site of serine 367 (R-A-SP-S-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17302

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  Connexin43 (phospho-Ser367) antibody detects endogenous levels of Connexin43 only when phosphorylated at serine 367.

• References:

  Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211 Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353, Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654 W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin43 (phospho-Ser367) antibody (E011258).

Western blot analysis of extracts from HeLa (Line 1, 4, 7 and 10), K562 (Line 2, 5, 8 and 11) and 293 (Line 3, 6, 9 and 12) cells, untreated or treated with PMA (1ηM 30min), using Connexin43 (Ab-367) antibody (E021250, Lane 1, 2, 3, 4, 5 and 6) and Connexin43 (phospho-Ser367) antibody (E011258, Lane 7, 8, 9, 10, 11 and 12).