• Catalog Number:

  E1A8223A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ephrin B1/B2 around the phosphorylation site of tyrosine 329 (P-V-YP-I-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P98172/P52799

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  CEK5 ligand; CEK5 receptor ligand; CEK5-L; CEL5-L; EFL-3; EFNB1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; Ephrin-B1 precursor; EPL2; EPLG2; kinase ephrin-B1; LERK-2; LERK2; STRA1; STRA1 protein

• Specificity Sensitivity:

  Ephrin B1/B2 (Phospho-Tyr329) antibody detects endogenous levels of Ephrin B1/B2 only when phosphorylated at tyrosine 329.

• References:

  Beckmann M.P., EMBO J. 13:3757-3762(1994). Davis S., Science 266:816-819(1994). Ross M.T., Nature 434:325-337(2005).

• All Sites:

  Human: Tyr329/316; Mouse: Tyr318/319; Rat: Tyr328/-

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ephrin B1/B2 (Phospho-Tyr329) antibody. Ephrin B1/B2 (Phospho-Tyr329) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.