• Catalog Number:

  E1A8233A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Inos around the phosphorylation site of tyrosine 151 (Q-Y-YP-G-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  P35228

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  EC 1.14.13.39; HEP-NOS; hepatocyte NOS; inducible NOS; nitric oxide synthase, inducible; NOS type II; NOS2; NOS2A

• Specificity Sensitivity:

  Inos (Phospho-Tyr151) antibody detects endogenous levels of Inos only when phosphorylated at tyrosine 151.

• References:

  Geller D.A., Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993). Sherman P.A., Biochemistry 32:11600-11605(1993). Charles I.G., Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).

• All Sites:

  Human: Tyr151; Mouse: Tyr145; Rat: Tyr151

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Inos (Phospho-Tyr151) antibody. Inos (Phospho-Tyr151) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.