Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 15 (R-A-SP-G-Q).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P16949
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
Stathmin 1 (phospho-Ser15) antibody detects endogenous levels of Stathmin 1 only when phosphorylated at serine 15.
References:
Larsson N, et al. (1999) Mol Cell Biol; 19(3): 2242-2250
Küntziger T, et al. (2001) Mol Biol Cell; 12(2): 437-448
Vadlamudi RK, et al. (2005) Mol Cell Biol; 25(9): 3726-3736
Vázquez-Boland JA, et al. (2001) Clin Microbiol Rev; 14(3): 584-640
Howell B, et al. (1999) Mol Biol Cell; 10(1): 105-118
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Stathmin 1 (phospho-Ser15) antibody (E011234).