• Catalog Number:

  E1A7205B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Rac1/CDC42 around the phosphorylation site of serine 71 (P-L-SP-Y-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P63000/P15153/P60763/P60953

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 2:10000

• Other Names:

  p21-Rac1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1

• Specificity Sensitivity:

  Rac1/CDC42 (Ab-71) antibody detects endogenous levels of total Rac1/CDC42 protein.

• References:

  Didsbury J., J. Biol. Chem. 264:16378-16382(1989). Drivas G.T., Mol. Cell. Biol. 10:1793-1798(1990). Matos P., Biochem. Biophys. Res. Commun. 277:741-751(2000).

Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using Rac1/CDC42 (Ab-71) antibody.