• Catalog Number:

  E011001-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 63 (L-T-SP-P-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P05412

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  c-Jun (phospho-Ser63) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 63.

• References:

  Wei W, et al. (2005). Cancer Cell. 8(1): 25-33. Sevilla A, et al. (2004). Oncogene.23 (55): 8950-8958. Li L, et al. (2004). J Biol Chem.279 (6): 4058-4065. Hurd C, et al. (2002). Oncogene.21 (14): 2154-2160.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (phospho- ser63) antibody (E011001).

UV - + Western blot analysis of extracts from NIH/3T3 cells using c-Jun (phospho-Ser63) antibody (E011001)