• Catalog Number:

  E011222-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13541

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I

• Specificity Sensitivity:

  4E-BP1 (phospho-Thr36)) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

• References:

  Gingras AC, et al. Genes Dev Nov. 2005 Feb 15. Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64. Gingras AC, et al.(1999) Genes Dev:13(11): 1422-37. Lal L, et al.(2005) Blood;105(4): 1669-7.

• All Sites:

  Human: Thr36 or Thr37; Mouse: Thr35; Rat: Thr35

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody (E011222).

Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (E021215, Lane 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (E011222, Lane 3 and 4).