• Catalog Number:

  E1A7197A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKCθ around the phosphorylation site of serine 676 (R-L-SP-F-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04759

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Alter Name:

  PRKCQ , Protein kinase C theta type

• Specificity Sensitivity:

  PKCθ (phospho-Ser676) antibody detects endogenous levels of PKCθ only when phosphorylated at serine 676.

• References:

  Kristof Van Kolen, et,al. (2006) FEBS J; 273: 1843-1854. Martin Villalba, et,al. (2002) J. Cell Biol; 157: 253. Jie Zhang, et,al. (2004) J. Biol. Chem; 279: 22118-22123. A. F. Castro, et,al. (1998) Am J Physiol Cell Physiol; 275: C113-C119.

• All Sites:

  Human: Ser676; Mouse: Ser676; Rat: Ser676

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCθ (phospho-Ser676) antibody.

Western blot analysis of extracts from JK cells untreated or treated with PMA (200nM, 30mins), using PKCθ (Ab-676) antibody (Line 1 and 2) and PKCθ (phospho-Ser676) antibody (Line 3 and 4).