• Catalog Number:

  E011216-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Pyk2 around the phosphorylation site of tyrosine 402 (D-I-YP-A-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q14289

• Applications:

  WB:1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Pyk2 (phospho-Tyr402) antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402.

• References:

  Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699 Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789 Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662 Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031 Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 (phospho-Tyr402) antibody (E011216).

Western blot analysis of extract from Jurkat cells, untreated or treated with PMA (1ng/ml, 5min), using Pyk2 (Ab-402) antibody (E021209, Lane 1 and 2) and Pyk2 (phospho- Tyr402) antibody (E011216, Lane 3 and 4).