• Catalog Number:

  E1A0001A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ζ around the phosphorylation site of serine 58 (R-S-SP-W-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P63104

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ

• Specificity Sensitivity:

  14-3-3 ζ (phospho-Ser58) antibody detects endogenous levels of 14-3-3 ζ only when phosphorylated at serine 58.

• References:

  Ying H. Shen Mol. Biol. Cell, Nov 2003; 14: 4721. David W. Powell, Mol. Cell. Biol., Aug 2003; 23: 5376 - 5387. Mackintosh. C, (2004) Biochem. J. 381, 329 - 42. Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875 - 84.

• All Sites:

  Human: Ser58; Mouse: Ser58; Rat: Ser58

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 ζ (phospho-Ser58) antibody.

Western blot analysis of extracts from NIH/3T3 cells treated with UV (30mins), using 14-3-3 ζ (phospho-Ser58) antibody.

PMA + - Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 ζ (phospho-Ser58) antibody.