• Catalog Number:

  E011198-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SMC1 around the phosphorylation site of serine 957 (G-S-SP-Q-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14683

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Alter Name:

  Chromosome segregation protein SmcB, DXS423E, KIAA0178, SB1.8/DXS423E protein, SM1A, SMC1A, SMC1L1, SMC1alpha protein, Sb1.8, Structural maintenance of chromosome 1-like 1 protein

• Specificity Sensitivity:

  SMC1 (phospho-Ser957) antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957.

• References:

  Yang XM. et al. (2004) Mol Cell Biol. 24(12): 5290-5303. Kim ST. et al. (2002) Genes Dev. 16(5): 560-570. Revenkova E.et al. (2001) Mol Cell Biol. 21(20): 6984-6998.

• All Sites:

  Human: Ser957; Mouse: Ser957

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1 (phospho-Ser957) antibody (E011198).

Western blot analysis of extract from K562 cells, untreated or treated with UV (20min), using SMC1 (Ab-957) antibody (E021190, Lane 1 and 2) and SMC1 (phospho-Ser957) antibody (E011198, Lane 3 and 4).