• Catalog Number:

  E011195-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q00613

• Applications:

  WB: 1:500~1:1000 IF: 1:100-200

• Specificity Sensitivity:

  HSF1 (Phospho-Ser307) Antibody detects endogenous levels of HSF1 only when phosphorylated at serine 307.

• References:

  Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4 Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026 Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

Western blot analysis of extract from HUVEC cell treated with TNF-a, using HSF1 (Phospho-Ser307) Antibody (E011195, Lane 1 and 2).