• Catalog Number:

  E011246-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27361

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200

• Specificity Sensitivity:

  p44/42 MAP Kinase (phospho-Tyr204) antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at tyrosine 204

• References:

  TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397 Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321 Hironobu Ihn et al.(2000) Immunology 165: 2149-2155

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p44/42 MAP Kinase (phospho-Tyr204) antibody (E011246).

Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase (Ab-204) antibody (E021238, Line 1 and 2) and p44/42 MAP Kinase (phospho- Tyr204) antibody (E011246, Line 3 and 4).