• Catalog Number:

  E011186-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  EGFR (phospho-Thr678) antibody detects endogenous levels EGFR only when phosphorylated at threonine 678.

• References:

  Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874 Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674 O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94(7): 3250-3255 Noguchi T, et al. (1994) Mol Cell Biol; 14(10): 6674-6682 Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720

Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR (Ab-678) antibody (E021193, Line 1 and 2) and EGFR (phospho-Thr678) antibody (E011186, Line 3 and 4).