• Catalog Number:

  E011181-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ζ around the phosphorylation site of serine 58 (R-S-SP-W-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P63104

• Applications:

  WB: 1:500-1000 IHC: 1:50-1:100 IF: 1:100-200

• Specificity Sensitivity:

  14-3-3 ζ (Phospho-Ser58) antibody detects endogenous levels of 14-3-3 ζ only when phosphorylated at serine 58.

• References:

  Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310 Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387 Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 ζ (Phospho-Ser58) antibody (E011181).

Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-α (20ng/ml, 5min), using 14-3-3 ζ (Ab-58) antibody (E021188, lane 1 and 2) and 14-3-3 ζ (Phospho- Ser58) antibody (E011181, lane 3 and 4).