Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of threonine 312 (L-A-TP-P-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q02078
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000 IP: Various Dilution
Specificity Sensitivity:
MEF2A (phospho-Thr312) antibody detects endogenous levels of MEF2A only when phosphorylated at threonine 312.
References:
Kato Y, et al. (2000) J Biol Chem. 275(24): 18534-18540.
Zhao M, et al. (1999) Mol Cell Biol. 19(1): 21-30.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A (phospho-Thr312) antibody.
Western blot analysis of extracts from NIH/3T3 cells treated with PMA, using MEF2A (phospho-Thr312) antibody.