• Catalog Number:

  E1A7185A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 6 (P-Q-SP-D-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  p53 (phospho-Ser6) antibody detects endogenous levels of p53 only when phosphorylated at serine 6.

• References:

  Thompson T, et al.(2004 ) J Biol Chem; 279(51): 53015-22. Jackson MW, et al. (2004 ) Oncogene. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934. Lohrum, M. and Scheidtmann, K.H. (1996) Oncogene 13, 2527-2539.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser6) antibody.

Western blot analysis using p53 (phospho-Ser6) antibody.