• Catalog Number:

  E011175-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLCγ2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16885

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  PLCγ2 (phospho-Tyr753) antibody detects endogenous levels of PLCγ2 only when phosphorylated at tyrosine 753.

• References:

  Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999 Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661 Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029 Rodriguez R, et al. (2003) Biochem J 374 : 269-280

Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 5min), using PLCγ2 (Ab-753) antibody (E021186, Lane 1 and 2) and PLCγ2 (phospho-Tyr753) antibody (E011175, Lane 3 and 4).