• Catalog Number:

  E1A0021A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 927 (C-D-SP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19838

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  DNA-binding factor KBF1, EBP-1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50, Nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit

• Specificity Sensitivity:

  NF-κB p105/p50 (phospho-Ser927) antibody detects endogenous levels of NF-κB p105/p50 only when phosphorylated at serine 927.

• References:

  Theresa Joseph, Am J Physiol Lung Cell Mol Physiol, Mar 2005; 288: L471 - L479. Sabine Kirchhoff, Eur. J. Biochem., Apr 1999; 261: 546. Gerald Thiel, Eur. J. Biochem., Jul 2004; 271: 2855 - 2862.

• All Sites:

  Human: Ser927; Mouse: Ser930

P-peptide - +

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105/p50 (phospho-Ser927) antibody. Western blot analysis of extracts from Hela cells treated with LPS (100ng/ml, 30min), using NF-κB p105/p50 (phospho-Ser927) antibody .

EGF + - Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NF-κB p105/p50 (phospho-Ser927) antibody.