• Catalog Number:

  E011167-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DNA PKcs around the phosphorylation site of threonine 2609 (V-E-TP-Q-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P78527

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  DNA PKcs (phospho-Thr2609) antibody detects endogenous levels of DNA PKcs only when phosphorylated at threonine 2609.

• References:

  Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338 Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848 Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251

Western blot analysis of extract from K562 cells untreated and treated with hydroxyurea, using DNA PKcs (Ab-2609) antibody (E021179, Lane 1 and 2) and DNA PKcs (phospho-Thr2609) antibody (E011167, Lane 3 and 4).