• Catalog Number:

  E011165-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 423 (R-S-TP-M-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13153//Q13177//O75914

• Applications:

  IHC: 1:50-1:100

• Specificity Sensitivity:

  PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) antibody detects endogenous levels of PAK1/PAK2/PAK3 only when phosphorylated at threonine 423/402/421.

• References:

  Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695. Jung JH, et al. (2005) J Biol Chem; 280:40025-40031. Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189 Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) antibody (E011165).