• Catalog Number:

  E1A1182A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Telomerase around the phosphorylation site of serine 227 (G-G-SP-A-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O14746

• Applications:

  IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  EC 2.7.7.49, EST2, HEST2, TCS1, TERT, TRT, Telomerase catalytic subunit, Telomerase reverse transcriptase

• Specificity Sensitivity:

  Telomerase (Phospho-Ser227) antibody detects endogenous levels of Telomerase only when phosphorylated at serine 227.

• References:

  Nakamura T.M., Science 277:955-959(1997). Meyerson M., Cell 90:785-795(1997). Wick M., Gene 232:97-106(1999).

• All Sites:

  Human: Ser227

P-peptide - +

Immunofluorescence analysis of HuvEC cells, using Telomerase (Phospho-Ser227) antibody. Telomerase (Phospho-Ser227) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.