• Catalog Number:

  E011156-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 1176 (T-Q-SP-F-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29474

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  eNOS (phospho-Ser1176) antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176.

• References:

  Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS (phospho-Ser1176) antibody (E011156).