• Catalog Number:

  E1A8364A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK5 around the phosphorylation site of threonine 182 (L-M-TP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q8IW41

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  EC 2.7.11.1; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPK5; MAPKAP kinase 5; p38-regulated/activated protein kinase; PRAK

• Specificity Sensitivity:

  MAPKAPK5 (Phospho-Thr182) antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at threonine 182.

• References:

  New L., EMBO J. 17:3372-3384(1998). Sudo T., Biochem. Biophys. Res. Commun. 269:521-525(2000). Dephoure N., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

• All Sites:

  Human: Thr182; Mouse: Thr182

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK5 (Phospho-Thr182) antibody. Western blot analysis of extracts from K562 cells, treated with Na3VO4 (0.3nM, 40mins), using MAPKAPK5 (Phospho-Thr182) antibody.