• Catalog Number:

  E011147-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of serine 516 (Q-P-SP-T-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  O96017

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Chk2 (phospho-Ser516) antibody detects endogenous levels of Chk2 only when phosphorylated at serine 516.

• References:

  Wu X, Chen J et al.(2003)J Biol Chem; 278(38): 36163-8 Schwarz JK, et al.(2003)Mol Cancer Res; 1(8): 598-609 Lou Z, et al.(2003) Nature; 421(6926): 957-61

Western blot analysis of extracts from HeLa cells using Chk2 (phospho-Ser516) antibody (E011147).