• Catalog Number:

  E011285-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human α- Synuclein around the phosphorylation site of tyrosine 133 (E-G-YP-Q-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P37840

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  α-Synuclein (phospho-Tyr133) antibody detects endogenous levels ofα-Synuclein only when phosphorylated at tyrosine 133.

• References:

  Kozikowski AP, et al. (2006)ChemMedChem. 1(2):256-66. Jakowec MW,et al.(2001)Dev Neurosci.23(2):91-9. El-Agnaf OM,et al.(1998)FEBS Lett. 440(1-2):67-70.

Immunofluorescence staining of methanol-fixed HeLa cells usingα-Synuclein (phospho-Tyr133) antibody (E011285, Red).