• Catalog Number:

  E011145-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MKK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  P46734

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200

• Specificity Sensitivity:

  MKK3 (phospho-Ser189) antibody detects endogenous levels of MKK3 only when phosphorylated at serine189.

• References:

  Wang W, et al. (2002)Mol Cell Biol ; 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using MKK3 (phospho-Ser189) antibody (E011145).

Western blot analysis of extract from MDA-MB-435 cells untreated or treated with UV, using MKK3 (Ab-189) antibody (E021116, Lane 1 and 2) and MKK3 (phospho-Ser189) antibody (E011145, Lane 3 and 4).