• Catalog Number:

  E1A0998A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 250 (E-L-SP-P-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q15796

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

   JV18-1; Mad-related protein 2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Smad 2

• Specificity Sensitivity:

  Smad2 (Phospho-Ser250) antibody detects endogenous levels of Smad2 only when phosphorylated at serine 250.

• References:

  Riggins G.J., Nat. Genet. 13-347-349(1996). Zhang Y., Nature 383-168-172(1996). Eppert K., Cell 86:543-552(1996).

• All Sites:

  Human: Ser250; Mouse: Ser250; Rat: Ser250

Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using Smad2 (Phospho-Ser250) antibody.