• Catalog Number:

  E011139-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23528

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  cofilin (phospho-Ser3) antibody detects endogenous levels of cofilin only when phosphorylated at serine 3.

• References:

  Kobayashi M, et al. (2006) EMBO J 25(4): 713-26. Wang Y, et al. (2005) Biol Chem 280(13): 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (phospho-Ser3) antibody (E011139).

Western blot analysis of extracts from COLO205 cells using cofilin (Ab-1022) antibody (E021164, Lane 1 and 2) and cofilin (phospho-Ser3) antibody (E011139, Lane 3 and 4).