• Catalog Number:

  E012092-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IKK-a/b around the phosphorylation site of serine 176 (Q-G-SP-L-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O15111/ O14920

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  IKK-a/b (Phospho-Ser176) antibody detects endogenous levels of IKK-a/b only when phosphorylated at serine 176.

• References:

  Mercurio F., et al. (1997) Science 278:860-866. Ling L., Cao Z., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:3792-3797. Dolcet X, et al. (2006) J Biol Chem 281, 22118-30. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.

Western Blot analysis of NIH-3T3 cells using IKK-a/b (Phospho-Ser176) Polyclonal Antibody diluted at 1：1000.

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using IKK-a/ b (Phospho-Ser176) Antibody (E012092).