• Catalog Number:

  E011137-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P98177

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197.

• References:

  Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46. Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using AFX (phospho-Ser197) antibody (E011137).

Western blot analysis of extracts from 293 cells using AFX (Ab-197) antibody (E021162, Lane 1 and 2) and AFX (phospho-Ser197) antibody (E011137, Lane 3 and 4).