• Catalog Number:

  E011218-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human β-Catenin around the phosphorylation site of serine 33 (L-D-SP-G-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35222

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  β-Catenin (phospho-Ser33) antibody detects endogenous levels of β-Catenin only when phosphorylated at serine 33.

• References:

  Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379 Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200 Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195 Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764 Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using β-Catenin (phospho-Ser33) antibody (E011218).

Western blot analysis of extracts from SW 626 cells , untreated or treated with Calyculin A (50nM, 30min), using β-Catenin (Ab-33) antibody (E021211, Lane 1 and 2) and β-Catenin (phospho-Ser33) antibody (E011218, Lane 3 and 4).