• Catalog Number:

  E011091-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P12931

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Src (phospho-Tyr418) antibody detects endogenous levels of Src only when phosphorylated at tyrosine 418.

• References:

  Dufilho M, et al. (2005) J Biol Chem. 280(43):35999-36006. Sanders MA, et al. (2005) J Biol Chem. 280(25): 23516-23522. Murugappan S, et al. (2005) Blood. 106(2):550-557. Gu JJ, et al. (2005) Blood. 105(8): 3270-3277.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Src (phospho- Tyr418) antibody (E011091).

Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody (E021115, Lane 1 and 2) and Src (phospho-Tyr418) antibody (E011091, Lane 3 and 4).