• Catalog Number:

  E1A7150A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Merlin around the phosphorylation site of serine 518 (R-L-SP-M-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35240

• Applications:

  WB: 1:500~1:1000 ELISA: 1:4000

• Alter Name:

  NF2 , MERL

• Specificity Sensitivity:

  Merlin (phospho-Ser518) antibody detects endogenous levels of Merlin only when phosphorylated at serine 518.

• References:

  Guang-Hui Xiao, et,al. (2005) Mol. Cell. Biol; 25: 2384-2394. Hi-Su Yang, et,al. (2006) Cancer Res; 66: 2708-2715. R Bohni, et,al. (1994) J. Biol. Chem; 269: 14541-14545. Adam J. Ratner, et,al. (2001) J. Biol. Chem; 276: 19267-19275.

• All Sites:

  Human: Ser518; Mouse: Ser518; Rat: Ser584

Western blot analysis of extracts from HuvEc cells untreated or treated with IFN-α (1000U/ml, 18 hours), using Merlin (Ab-518) antibody (Line 1 and 2) and Merlin (phospho-Ser518) antibody (Line 3 and 4).