• Catalog Number:

  E011088-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IGF-1R around the phosphorylation site of tyrosine 1165/tyrosine 1166 (T-D-YP-YP-R-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08069

• Applications:

  WB: 1:500~1:1000 IF:1:100~1:200

• Specificity Sensitivity:

  IGF-1R (phospho-Tyr1165/Tyr1166) antibody detects endogenous levels of IGF-1R only when phosphorylated at tyrosine 1165/tyrosine1166.

• References:

  Li S, et al. (1994) J Biol Chem; 269(51): 32558-64.

293 | SK-OV3 | | 293 | Western blot analysis using IGF-1R (Ab-1165/1166) antibody (E021081, Line 1, 2 and 3) and IGF-1R (phospho- Tyr1165/Tyr1166) antibody (E011088, Line 4 and 5).

Immunofluorescence staining of methanol-fixed MCF7 cells using IGF-1R (phospho-Tyr1165/Tyr1166) antibody (E011088, Red).