• Catalog Number:

  E011085-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1214 (F-H-YP-D-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35968

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  VEGFR2 (phospho-Tyr1214) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1214.

• References:

  Lamalice L, et al. (2004). Oncogene. 23(2): 434-445. Takahashi T, et al. (2001). EMBO J .20(11): 2768-2778.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using VEGFR2 (phospho-Tyr1214) antibody (E011085).

P-Peptide - + Western blot analysis of extracts from SKOV3 cells using VEGFR2 (phospho-Tyr1214) antibody (E011085).