• Catalog Number:

  E011253-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16539

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  p38 MAPK (phospho-Tyr182) antibody detects endogenous levels of P38MAPK only when phosphorylated at tyrosine 182.

• References:

  Ming Zheng, et al.(2005) The FASEB Journal. 19: 109-111 Bernt van den et al.(2001) Blink Immunology, 166: 582-587 Arshad Rahman, et al. (2004) Am J Physiol Lung Cell Mol Physiol 287: L1017-L1024 Osamu Yoshino, et al. (2003) Endocrinology & Metabolism Vol. 88: 2236-2241

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using P38 MAPK (phospho- Tyr182) antibody (E011253).

Western blot analysis of extracts from NIH-3T3 (Line 1, 4, 7 and 10) and cos7 (Line 2, 5, 8 and 11 and K562 (Line 3, 6, 9 and 12) cells, untreated or treated with UV (20min), using P38 MAPK (Ab-182) antibody (E021245, Lane 1, 2, 3, 4, 5 and 6) and P38 MAPK (phospho- Tyr182) antibody (E011253, Lane 7, 8, 9, 10, 11 and12).