• Catalog Number:

  E011080-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of serine 1070 (R-Y-SP-S-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  EGFR (phospho-Ser1070) antibody detects endogenous levels of EGFR only when phosphorylated at serine1070.

• References:

  Feinmesser RL,et al. (1999) J Biol Chem; 274(23): 16168-73. Gamou S,et al. (1995) FEBS Lett; 357(2): 161-4. Gamou S, et al. (1994) J Cell Physiol; 158(1): 151-9. Heisermann GJ, et al. (1988) J Biol Chem; 263(26): 13152-8.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using EGFR (phospho-Ser1070) antibody (E011080).

Western blot analysis of extract from SK-OV3 cells untreated or treated with EGF using EGFR (Ab-1070) antibody (E021073, Lane 1 and 2) and EGFR (phospho- Ser1070) antibody (E011080, Lane 3 and 4).